What is the standard sequence for instrument preparation prior to sterilization?

• A. Pre-clean and remove debris, then load into sterilizer following packaging guidelines.
• B. Load first, then clean.
• C. Disinfect and dry only.
• D. Sterilize without cleaning.

Answer: (A)

The standard sequence for instrument preparation before sterilization starts with cleaning to remove debris and organic material, because any residue can shield microorganisms and prevent the sterilant from penetrating effectively. After cleaning, instruments should be loaded or packaged according to the sterilizer’s guidelines so that the sterilant can circulate properly and the integrity of the packs is maintained for future use.

Loading before cleaning would trap debris and hinder sterilization. Disinfecting and drying alone do not achieve true sterilization, and sterilizing without cleaning leaves material that can protect microbes from the sterilant.